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Biology and Microbiology Lab

Laboratorio de Biología y Microbiología

IMDEA Water hosts a biological laboratory providing analysis on surface and groundwater to:

  • Support monitoring programs of water quality by means of national and international standardized methods and in agreement with WFD 2000/60/CE y GWD 2006/118/CE.

  • Monitor cyanobacterial blooms and their toxins in surface water and to develop technological solutions for removal of cyanotoxins during water treatment.

  • Monitor crustacean communities from groundwater and groundwater dependent ecosystems (hyporheic zone of rivers and streams) to provide an integrated assessment of groundwater systems

Applications

  • Microinvertebrate samplingGroundwater and groundwater dependent ecosystems (GDEs) (i.e. hyporheic zone of rivers) monitoring and biodiversity assessment.
  • Distribution of stygobites invertebrates in groundwater ecosystems (inland and coastal aquifers).
  • Assessment of groundwater ecosystems health 

  • Surface water bodies monitoring (rivers, lakes, rivers) based on biological indicators (invertebrates)
  • Specimens identification of cyanobacteria, cyclopoids and ostracods to the lowest taxonomic level rank
  • Aquacultures and ecotoxicology tests (bioassays, chronic, acute and sublethal assays) for and integrated biological monitoring of water quality supporting physico-chemical and bacteriological tests.
  • Biodegradation of cyanotoxins and diversity of biodegrading bacterial populations using classic gene markers and metagenomics studies.
  • Developing molecular tools for monitoring cyanobacterial blooms and their toxins in surface water.
  • Developing technological solution for biological monitoring and elimination of cyanotoxins during water treatment.
  • Toxicity tests with aquatic organisms at the individual, population and community level (using micro-/mesocosms).

Equipment

  • Ecohydrology unit: the microbiological unit is equipped with fully high-quality optical microscopes systems available with high-resolution photo-montage systems for invertebrate’s identifications and aquaculture:
    • Light microscope, Olympus CX41
    • Stereo microscope, Olympus SZX10
    • Stereo microscope, Olympus SZX7
    • Stereo microscope, Olympus SZ51
    • Digital photo camera, Olympus
    • Incubator for groundwater invertebrates
    • Aquacultures aquariums  
  • Cyanobacteria and cyanotoxins unit:
    • Incubator for cyanobacteria and bacteria cultures
    • Rotary evaporator with a cooling system, Buchi, for extraction of cyanotoxins
    • Solid Phase extraction equipment, for concentrating toxin extracts.
  • Molecular biology unit:
    • Gel Documentation System, AlphaImager, for documentation of gel electropheresis
    • Real Time PCR machine, AB7300, for quantitative and qualitative gene studies
    • Nano-photometer, Epoch, for measuring DNA concentration in as low as 2 µl volume.
    • Gel Electrophoresis Equipment, Biorad, for electrophoresis of DNA and RNA
    • PCR Thermocycler, to perform PCR reaction
    • Homogenizer for DNA extraction, Precellys, to extract DNA from bacterial cells.
  • Field equipment for ecological monitoring of rivers, hyporheic zone and groundwater:
    • Grundfos pump for biological survey in piezometers
    • Bou Rouch pump for biological survey of the hyporheic zone
    • PVC Minipiezometers for semi-permanent monitoring of the hyporheic zone
    • Artificial substrates and baited traps
    • Sediment core Cobra
    • Bailers for boreholes water sampling
    • Water quality instruments Hanna with sensors for temperature, conductivity, pH y dissolved oxygen
    • Data loggers for temperature (Gemini Tinytag Explorer)
    • Electric contact gauge
    • GPS
    • Sieves  
    • Planktonic reds to sample surface and benthic invertebrates (grabs, dredges, box-corers and hand nets) as well as planktonic cyanobacteria
    • Planktonic reds to sample for groundwater invertebrates survey (Cvetkov net for shallow wells, borehole net)

 

PCR Thermocycler, to perform PCR reaction Gel Documentation System, AlphaImager, for documentation of gel electropheresis

PCR Thermocycler, to perform PCR reaction

Gel Documentation System, AlphaImager, for documentation of gel electropheresis

Incubator for cyanobacteria and bacteria cultures

Incubator for cyanobacteria and bacteria cultures

 

Scientific & technical offer: Biology and microbiology laboratory