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Biology and Microbiology Lab

Laboratorio de Biología y Microbiología

The Biology and Microbiology Laboratory supports water quality monitoring programs through standardized and innovative methods to achieve the objectives set by the Water Framework Directive (DMA 2000/60 / EC)

Applications

  • Monitoring of cyanobacteria blooms and their toxins (microcystins) in surface waters
  • Analysis of microbiological indicators (E. coli, Enterococcos, Coliforms, Clostridium, Pseudomonas aeruginosa) to determine the quality of drinking and recreational waters
  • Analysis of biological indicators for the determination of the ecological state of the waters
  • Determination of Minimum Inhibitory Concentrations (MICs) of microorganisms (antibiograms)
  • Acute and chronic toxicity tests with algae, invertebrates and fish following standard protocols (ISO, OECD)
  • Microcosm and mesocosm tests at the population and community level

Equipment

  • Microscopy unit: the microscopy unit is equipped with high-quality optical microscopes, with high-resolution photo-mounting systems, to facilitate identification and counting microscopic organisms:
    • Light microscope, Olympus CX41
    • Stereo microscope, Olympus SZX10
    • Stereo microscope, Olympus SZX7
    • Stereo microscope, Olympus SZ51
    • Digital photo camera, Olympus 
  • Cyanobacteria and Cyanotoxins Unit:
    • Incubator for cyanobacteria and bacteria cultures
    • Rotary evaporator with a cooling system, Buchi, for extraction of cyanotoxins
    • Solid Phase extraction equipment, for concentrating toxin extracts.
  • Molecular Biology Unit:
    • Electrophoresis gels documentation system AlphaImager
    • Real-time PCR (AB7300) for quantitative and qualitative gene studies
    • Nano-photometer (Epoch) for the quantification of DNA and RNA
    • Gel electrophoresis equipment (Biorad) for DNA and RNA
    • Thermocycler (PCR) for DNA amplification
    • Homogenizer for DNA extraction (Precellys)
  • Ecotoxicology Unit:
    • Incubators for testing under standard conditions
    • Cultures of algae, invertebrates and fish
    • Tanks of different sizes for standard and microcosm tests
    • Bathtubs with automatic temperature and lighting regulation
  • Field equipment for ecological monitoring of rivers:
    • Equipment for measuring temperature, conductivity, dissolved oxygen and pH in situ
    • Temperature data loggers (Gemini Tinytag Explorer)
    • Electrical contact indicator
    • GPS
    • Screens and networks for macroinvertebrate and phytoplankton sampling
    • Flowmeter
PCR Thermocycler, to perform PCR reaction Gel Documentation System, AlphaImager, for documentation of gel electropheresis

PCR Thermocycler, to perform PCR reaction

Gel Documentation System, AlphaImager, for documentation of gel electropheresis

Incubator for cyanobacteria and bacteria cultures

Incubator for cyanobacteria and bacteria cultures

 

Analytical services