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Biology and Microbiology Lab

Laboratorio de Biología y Microbiología

The Biology and Microbiology Laboratory supports water quality monitoring programs through standardized and innovative methods to achieve the objectives set by the Water Framework Directive (DMA 2000/60 / EC)

Applications

  • Monitoring of cyanobacteria blooms and their toxins (microcystins) in surface waters
  • Analysis of microbiological indicators (E. coli, Enterococcos, Coliforms, Clostridium, Pseudomonas aeruginosa) to determine the quality of drinking and recreational waters
  • Analysis of biological indicators for the determination of the ecological state of the waters
  • Determination of Minimum Inhibitory Concentrations (MICs) of microorganisms (antibiograms)
  • Acute and chronic toxicity tests with algae, invertebrates and fish following standard protocols (ISO, OECD)
  • Microcosm and mesocosm tests at the population and community level

Equipment

  • Microscopy unit: the microscopy unit is equipped with high-quality optical microscopes, with high-resolution photo-mounting systems, to facilitate identification and counting microscopic organisms:
    • Light microscope, Olympus CX41
    • Stereo microscope, Olympus SZX10
    • Stereo microscope, Olympus SZX7
    • Stereo microscope, Olympus SZ51
    • Digital photo camera, Olympus 
  • Cyanobacteria and Cyanotoxins Unit:
    • Incubator for cyanobacteria and bacteria cultures
    • Rotary evaporator with a cooling system, Buchi, for extraction of cyanotoxins
    • Solid Phase extraction equipment, for concentrating toxin extracts.
  • Molecular Biology Unit:
    • Electrophoresis gels documentation system GelDoc Go Imaging System of Bio-Rad
    • Real-time PCR (AB7300) for quantitative and qualitative gene studies
    • Nano-photometer (Epoch) for the quantification of DNA and RNA
    • Gel electrophoresis equipment (Biorad) for DNA and RNA
    • Thermocycler (PCR) for DNA amplification
    • Homogenizer for DNA extraction (Precellys)
  • Ecotoxicology Unit:
    • Incubators for testing under standard conditions
    • Cultures of algae, invertebrates and fish
    • Tanks of different sizes for standard and microcosm tests
    • Bathtubs with automatic temperature and lighting regulation
  • Field equipment for ecological monitoring of rivers:
    • Equipment for measuring temperature, conductivity, dissolved oxygen and pH in situ
    • Temperature data loggers (Gemini Tinytag Explorer)
    • Electrical contact indicator
    • GPS
    • Screens and networks for macroinvertebrate and phytoplankton sampling
    • Flowmeter
PCR Thermocycler, to perform PCR reaction GelDoc Go Imaging System de Bio-Rad

PCR Thermocycler, to perform PCR reaction

Gel Documentation System, GelDoc Go Imaging System of Bio-Rad

Incubator for cyanobacteria and bacteria cultures

Incubator for cyanobacteria and bacteria cultures

 

Analytical services